MountainsMap® Example Applications

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Bio-fuels research – number & size of fat pockets stored in bacteria

AFM-IR (atomic force microscope with tunable pulsed IR source) + MountainsMap® Premium software

Input Data

  • AFM topography of bacterium filament combined with IR absorption image highlighting fat pockets.
  • Results

  • Overlay of IR absorption image on 3D surface topography making it possible to observe of fat pockets at different zoom levels and angles.
  • Fat pocket count and calculation of morphological parameters to quantify pocket size.
  • Mountains® tools used

  • Image overlay on 3D topography.
  • Advanced filtering.
  • Motifs analysis in Grains & Particles module.

  • Images courtesy of A.Dazzi and A. Deniset-Besseau, Laboratoire de Chimie Physique, Université Paris-Sud, Orsay, France.

    Read more . . .

    Surface Newsletter November 2013
    Article in the Journal of Physical Chemistry

    List of applications in gallery

    3D Optical

    3D Optical Profilers

    3D Scanning

    3D Scanning Profilometers


    AFM (atomic force microscope)


    SEM (scanning electron microscope)


    Hyperspectal microscopes


    2D Profilometers

    Correlative Studies

    3D Optical Profiler + SEM (scanning electron microscope)

    AFM-IR (atomic force microscope with tunable pulsed infrared source)

    STM (scanning tunneling microscope) + SEM (scanning electron microscope)

    AFM (atomic force microscope) + SEM (scanning electron microscope)

    AFM (atomic force microscope) + fluorescence microscope

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